Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FSAF1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C1orf131 sometimes referred to as Family with Sequence Similarity 40 Member B (FAM40B) is a protein that plays a role in various cellular mechanisms. It has a mass of approximately 119 kDa. This protein is expressed in different tissues throughout the body with notable expression in the brain. Research indicates its action may involve roles in cellular structures or signaling due to its widespread distribution.
Biological function summary
C1orf131 serves a functional role linked to cellular processes and regulatory mechanisms. It might belong to a complex that regulates intracellular signaling pathways although specific interactions remain to be fully clarified. Its broad expression suggests functions in basic cellular activities possibly affecting cell growth and communication. As scientists uncover more understanding the exact biological roles of C1orf131 continues to expand.
Pathways
C1orf131 may integrate into broad signaling networks that control cellular proliferation and maintenance. One potential interaction is with the PI3K/Akt pathway an important regulatory pathway for cell survival and metabolism. Within this pathway C1orf131 could have associative functions connecting with regulatory proteins like the AKT1. Connections to these pathways suggest C1orf131’s involvement in cellular homeostasis and adaptation.
The involvement of C1orf131 has been noted in certain cases of neurological disorders. Research points to its potential role in neurodegenerative conditions possibly through interactions with other proteins involved in neurodegeneration like Tau. Understanding its interactions and mechanisms in these disorders might provide insights into potential therapeutic targets. The connection to disorders highlights the importance of further investigations into C1orf131’s biological significance.
