Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-RIMOC1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C5orf51 also known as Chromosome 5 open reading frame 51 is a lesser-known protein that has a molecular weight of approximately 32 kDa. This protein is mainly expressed in the testis but lower levels of expression can be found in other tissues. It is a non-enzymatic protein indicating its role might not involve catalytic activity but possibly structural or regulatory functions. Because of its expression pattern it has been minimally characterized yet scientists consider it an area open for further exploration.
Biological function summary
C5orf51 plays a role that is not yet fully understood but researchers suspect it might be involved in regulating cellular processes in the tissues it is expressed in. While it is not currently identified as part of a larger protein complex its localization hints at potential interactions within the cellular environment. Much work remains to determine its exact biological functions and interactions.
Pathways
Current research suggests that C5orf51 could intersect with pathways involved in reproductive or germ cell function due to its primary expression in the testis. Scientists postulate potential interactions with proteins involved in spermatogenesis though these associations are largely speculative. Pathway analysis continues to be an important step in uncovering the broader role of this protein.
Scientists have yet to establish direct links between C5orf51 and specific diseases. However its expression profile implies a potential connection to reproductive health such as infertility. Researchers continue investigating if C5orf51 interacts with proteins implicated in testis-related disorders searching for evidence that may illuminate its impact on human health. As studies progress additional associations with other conditions could emerge.
