Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-C6orf62, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C6orf62 also known as Chromosome 6 Open Reading Frame 62 is a protein of roughly 36 kDa. This protein plays a mechanical role related to cell cycle regulation and is expressed mainly in tissues like liver and kidney. It does not act alone; instead it often interacts with other cellular components influencing cellular processes through its interactions.
Biological function summary
The function of this protein involves regulation of cell proliferation and apoptosis. C6orf62 is not a single-acting entity but is thought to be part of a larger protein complex. This association allows it to maintain normal cellular functions by modulating expression levels of genes important for growth and programmed cell death.
Pathways
C6orf62 has key roles in the cell cycle and apoptotic pathways. It associates with proteins like cyclins and caspases. Cyclins are involved in progression through different phases of the cell cycle while caspases are important for the initiation of apoptosis. C6orf62 helps balance these pathways depending on the cellular context ensuring proper function and survival of the cell.
Studies suggest connections of C6orf62 with cancers and neurodegenerative disorders. Dysregulation of this protein may affect its associated pathways leading to uncontrolled cell growth as seen in certain cancers or improper cell death in neurodegenerative diseases. Through its connections in these pathways C6orf62 also potentially interacts with oncogenes and neuroprotective proteins impacting disease progression and pathology.
