Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-CACNB3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CAB3 also known as calcineurin B homologous protein 3 (CaCNB3) is a protein involved in intracellular signaling. The protein weighs approximately 22 kDa and expresses in various tissues including the heart and brain. Functionally CAB3 participates in calcium-binding and signaling processes facilitating interactions essential for cellular responses to changes in calcium levels.
Biological function summary
CAB3 plays an integral role in calcium-dependent signaling pathways. This protein functions as part of a larger calcineurin complex alongside calcineurin A. CAB3 and the complex it forms modulate the activity of several downstream targets influencing cellular processes like gene expression and cytoskeletal dynamics. In neurons CAB3 contributes to the synaptic plasticity necessary for learning and memory.
Pathways
CAB3 is an important component in calcium and NFAT signaling pathways. It directly regulates these pathways impacting gene expression and cellular development. CAB3 interacts with other proteins like calmodulin which facilitates its binding and functional actions within the pathway. Through modulation of these pathways CAB3 participates in orchestrating cellular responses to external stimuli.
Mutations or dysregulation of CAB3 relate to cardiac hypertrophy and neurodegenerative diseases such as Alzheimer’s. Misregulation of CAB3 activity can disrupt calcium homeostasis aggravating disease processes. The connection to calcineurin is significant as alterations in this relationship could influence the progression of these disorders offering potential targets for therapeutic intervention.
