Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-CCDC137, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The CC137 protein also known as C-C motif chemokine ligand 13 (CCL13) functions mechanically as a cytokine involved in immune system signaling. This protein has a molecular mass of approximately 10 kDa. It is mainly expressed in immune cells including macrophages monocytes and lymphocytes. The CC137 protein can induce chemotaxis directing the movement of immune cells to sites of inflammation and infection.
Biological function summary
CC137 is essential for modulating immune responses and is part of the chemokine family which regulates the migration of leukocytes. CC137 can form part of a larger cytokine signaling complex enhancing the communication between immune cells. It is involved in the regulation of inflammatory responses by activating specific receptors on the surface of target cells which leads to the activation of intracellular signaling pathways.
Pathways
CC137 participates in the chemokine signaling pathway and the JAK-STAT signaling pathway. It plays a role in the recruitment and activation of immune cells through interactions with chemokine receptors like CCR2 and CCR3. These pathways are important for movements and activation of various immune cell types linking CC137 to proteins such as JAK2 and STAT3 which are integral to signal transduction processes.
CC137 has been implicated in inflammatory diseases and allergic responses. It is connected to asthma through its role in promoting eosinophil movement and activation. Additionally CC137 is linked to rheumatoid arthritis where aberrant expression contributes to chronic inflammation within joints. In these diseases CC137 can interact with TNF-alpha a critical cytokine related to inflammation and immune responses.
