Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-EI24, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
EI24 also known as PIG8 is an apoptosis-inducing factor which has a molecular weight of approximately 24 kDa. It functions mechanically by inducing programmed cell death and is involved in the p53 pathway which is associated with tumor suppression. EI24 is widely expressed in various tissues with higher expression observed in the thymus lymph nodes and spleen.
Biological function summary
The activity of EI24 is significant in regulating apoptosis. It contributes to cellular homeostasis by facilitating the elimination of damaged or unneeded cells. EI24 does not operate as part of any known large protein complexes but it interacts with proteins related to the cell cycle and apoptosis regulation. By promoting apoptosis EI24 helps maintain normal cellular functions and prevents the accumulation of defective cells.
Pathways
Several important biological activities involve EI24. It plays a role in the p53-mediated apoptotic pathway which is critical for responding to DNA damage and other cellular stresses. In this pathway EI24 interacts with the tumor suppressor protein p53 promoting cell death in response to DNA damage. EI24 also associates with Bcl-2 family proteins which influence the apoptotic process. This association suggests a role for EI24 in promoting apoptosis through mitochondrial pathways.
EI24 is linked to certain types of cancer including breast cancer and lymphomas. Altered expression or mutations in the EI24 gene can contribute to tumorigenesis and cancer progression. Studies show that the loss of EI24 function interacts with p53 mutations influencing tumor development and resistance to apoptosis. The role of EI24 in cancer highlights its potential as a target for therapeutic interventions aiming to restore the apoptotic pathways in cancer cells.
