Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-RTL8C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CXX1 also known as Cytoskeletal X-linked Protein 1 functions as a structural component involved in maintaining cell integrity and stability. This protein weighs approximately 45 kilodaltons. Scientists predominantly observe CXX1 expression in epithelial tissues but it is also present in other cell types. The presence of CXX1 in various tissues suggests it might have diverse roles related to cellular structure and dynamics.
Biological function summary
CXX1 contributes to cellular shape and motility by connecting the actin cytoskeleton to specific membrane proteins facilitating intracellular signaling and transport. It often functions as part of large protein complexes that include actin-binding proteins. These interactions allow CXX1 to contribute to the dynamic rearrangement of cytoskeletal elements necessary for processes like cell migration and adhesion.
Pathways
CXX1 plays a role in the regulation of the cytoskeletal remodeling pathway and the Rho-GTPase signaling pathway. These pathways are essential for cell movement and shape adjustments. In the cytoskeletal remodeling pathway CXX1 interacts with proteins like RhoA which modulate actin dynamics. Through its involvement in these pathways CXX1 can impact cellular responses to external stimuli.
Defects or dysregulation of CXX1 associate with certain types of cancer and neurodegenerative disorders. Altered expression or mutation of CXX1 can contribute to tumor cell metastasis as improper cytoskeleton function may enhance cellular migration and invasion capacity. Additionally connections between CXX1 and Alzheimer's Disease arise via its interaction with Tau protein another key player in maintaining neuronal cell structure. This suggests that CXX1 might influence disease progression through its cytoskeletal roles.
