Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon12.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FOCAD, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Focadhesin also known as FDN-1 is a structural protein involved in cell adhesion processes. It has a molecular mass of approximately 50 kDa. Focadhesin primarily expresses in epithelial tissue where it plays a role in maintaining cell junction integrity. Researchers prefer focusing on this target for studies related to tissue development and repair. The solid expression in epithelial cells makes it integral to tissue architecture and stability.
Biological function summary
Focadhesin contributes to cellular adhesion and communication mechanisms. It operates as part of a multiprotein complex associated with adhesion molecules such as cadherins and integrins. This complex facilitates the stabilization of cell junctions supporting structural cohesion between cells. Focadhesin's presence is necessary for processes involving epithelial cell cohesion and barrier function. Its interactions with other adhesion molecules promote the assembly and maintenance of intercellular connections.
Pathways
Focadhesin plays a significant role in cell signaling pathways that control cell adhesion and migration particularly the Wnt and PI3K/AKT pathways. Its interaction with proteins like beta-catenin and phosphoinositide 3-kinase highlights its importance in signaling cascades that affect cellular dynamics and growth. These pathways influence cellular responses and integrity impacting processes like tissue remodeling and stress responses.
Focadhesin's dysfunctional expression has links to epithelial cancers such as breast and colorectal cancer. Altered focadhesin levels may disrupt adhesion and signaling promoting tumor progression and metastasis. It also associates with fibrotic disorders like pulmonary fibrosis where improper adhesion molecule regulation contributes to structural tissue changes. Focadhesin's relationship with proteins such as E-cadherin and TGF-beta reveals its involvement in these pathological conditions making it a target of interest for therapeutic interventions.
