Description
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon4.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GLI4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GLI4 also known as Zinc Finger Protein GLI4 acts as a transcription factor involved in regulating gene expression. This protein has an approximate mass of 110 kDa. It is expressed in various tissues including the brain and spinal cord indicating its involvement in neural functions. GLI4 contains zinc finger motifs which are important for its DNA-binding capabilities allowing it to influence cell differentiation and proliferation.
Biological function summary
GLI4 plays significant roles in the development and maintenance of the central nervous system. It operates as part of the hedgehog signaling pathway acting alongside other GLI proteins as an important regulatory element. The interaction with DNA allows GLI4 to modulate signals that govern cell fate growth and division. It forms part of a transcription complex that selectively activates or represses genes affecting the expression profiles necessary for various developmental processes.
Pathways
GLI4 integrates itself into the hedgehog signaling pathway and Wnt signaling pathway. Within the hedgehog pathway it regulates various other proteins such as PTCH1 and SMO that are important for signal transduction. The involvement of GLI4 in these pathways is vital for directing cellular responses necessary for embryonic pattern formation and tissue regeneration. It interacts with other GLI family members including GLI1 and GLI2 coordinating fundamental developmental signals.
GLI4 relates to conditions such as medulloblastoma and basal cell carcinoma. Aberrant expression or mutation of GLI4 can contribute to the pathogenesis of these cancers through dysregulated hedgehog signaling. The overexpression of GLI4 often correlates with increased tumor growth and progression. It is also linked with proteins like SHH and PTCH1 where disruptions in their function due to GLI4 anomalies can exacerbate disease states.
