Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GTF3C6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GTF3C6 also known as Transcription Factor IIIC subunit 6 is a component of the transcription factor IIIC (TFIIIC) complex. It weighs approximately 78 kilodaltons and plays a mechanical role in the transcription process. GTF3C6 facilitates the assembly of the TFIIIC complex on DNA enabling the recruitment of RNA polymerase III. The protein is expressed in various tissues reflecting its role in basic cellular functions.
Biological function summary
The protein contributes to the transcription of small non-coding RNA genes such as 5S rRNA and tRNAs. GTF3C6 is a subunit of the TFIIIC complex and its function involves the recognition of specific promoter elements on DNA which is imperative for initiating transcription. By participating in the recognition and structuring of promoter regions GTF3C6 ensures proper transcription activation necessary for cell growth and metabolism.
Pathways
The engagement of GTF3C6 in RNA polymerase III transcription is significant. It operates within the RNA polymerase III transcription pathways interacting with TFIIIB and RNA polymerase III which are key components of the transcription machinery. GTF3C6 plays a role in the complex network of transcription regulation ensuring the timely transcription of essential RNAs that regulate protein synthesis and cell cycle progression.
Altered expression or dysfunction of GTF3C6 may associate with certain types of cancers. Given its role in transcription regulation deviations in GTF3C6 activity can disrupt normal cell proliferation processes. Association between GTF3C6 and other proteins in the RNA polymerase III pathway such as TFIIIB has shown relevance in studies of oncogenesis and potential cancer treatment avenues. Understanding these relationships can open doors to targeted therapies for diseases that involve dysregulation of transcription.
