Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 31 bp deletion in exon2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HMGN4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HMGN4 also known as High Mobility Group Nucleosome Binding Domain 4 is a chromosomal protein with an approximate mass of 12 kDa. This protein functions mechanically by binding to nucleosomes altering the chromatin structure and influencing gene expression. It is expressed in various tissues with higher levels found in the brain and testis. HMGN4 plays roles in regulating the dynamics of chromatin impacting accessibility and stability of DNA.
Biological function summary
HMGN4 modulates transcriptional activity by altering the chromatin compaction state not working as a part of a complex. It influences how genes are turned on or off impacting cellular processes such as differentiation and proliferation. This modulation occurs through its interaction with nucleosomes positioning it as an important factor in the control of gene regulatory mechanisms across different cell types.
Pathways
Researchers study HMGN4 to understand its involvement in transcriptional regulation and DNA repair pathways. These pathways guide critical cellular activities affecting how cells respond to damage and manage genetic information. HMGN4 interacts with proteins like histone H1 an important player in facilitating chromatin remodeling which supports effective gene regulation and maintains genomic integrity.
Researchers associate HMGN4 with certain types of cancer and neurological disorders. Aberrant expression levels of HMGN4 have links to tumor progression as altered chromatin states may lead to deregulation of oncogenes. The protein connects to Alzheimer's disease through its influence on neuronal gene expression implicating interactions with tau proteins. These associations highlight HMGN4 as a potential target for therapeutic interventions.
