Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon6 and 5 bp deletion in exon6.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HTR3D, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The 5HT3D Receptor also known as 5-Hydroxytryptamine Receptor 3D is a subtype of serotonin receptor. It is part of the ligand-gated ion channel family. This receptor is involved in mediating fast synaptic transmission in the central and peripheral nervous systems. The 5HT3D Receptor has a molecular mass of approximately 56 kDa. Expression of this receptor occurs in human brains and the gastrointestinal tract where it performs its role in neuronal signaling.
Biological function summary
The 5HT3D Receptor functions as a component of the ligand-gated ion channel complex allowing the passage of cations including sodium (Na+) and calcium (Ca2+) upon serotonin binding. This action affects neuronal excitability and neurotransmitter release. The receptor plays a significant role in intestinal motility modulating gut motility and in the central nervous system where it contributes to emotions and cognitive processes.
Pathways
The 5HT3D Receptor is involved in the serotonergic synapse pathway which is important for transmitting signals in the nervous system. The receptor works alongside other serotonin receptors such as 5HT3A influencing neurotransmitter release and neuronal excitability. It also interacts within the GABAergic synapse pathway where it may affect inhibitory neurotransmission alongside the GABA receptors. These interactions can impact mood regulation and cognitive functions.
The 5HT3D Receptor has associations with conditions such as irritable bowel syndrome (IBS) and anxiety disorders. Abnormal expression or function of this receptor can disrupt gastrointestinal motility leading to IBS symptoms. In the context of anxiety disorders the receptor’s interaction with neurotransmitter pathways can influence anxiety levels. Additionally it connects with other proteins such as serotonin transporter (SERT) and GABA receptors which are implicated in anxiety regulation.
