Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 28 bp deletion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-IFNE, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein IFNE also known as Interferon epsilon plays an important role in the immune response. It has a molecular mass of about 22-24 kDa and is mostly expressed in mucosal tissues particularly in the reproductive tract. Unlike other interferons IFNE expression does not get induced during infection reflecting its unique role in immune regulation. Scientists recognize it for its involvement in antiviral and antibacterial defense mechanisms at epithelial surfaces.
Biological function summary
IFNE contributes to the innate immune system by modulating immune responses without being part of a larger protein complex. It activates signaling pathways that result in the production of other immune response mediators. This protein supports the barrier function of epithelial cells enhancing the organism's ability to fend off infectious agents. Additionally it helps maintain homeostatic conditions in mucosal tissues by managing inflammation.
Pathways
IFNE integrates into the broader interferon signaling pathway and the JAK-STAT signaling pathway. These pathways mediate various cellular responses to external stimuli including pathogen infections. IFNE interacts with sister proteins such as IFN-alpha and IFN-beta in these pathways to drive protective responses and regulate inflammation. Understanding its pathway involvement assists researchers in designing targeted therapies for immune modulation.
Studies indicate that IFNE might influence conditions like cervical cancer and recurrent viral infections. Research shows its interaction with HPV-related proteins affects the progression of cervical cancer. In viral infections IFNE works with IFN-alpha to mediate the immune response reducing viral load and potentially altering disease outcomes. This makes it a point of interest in the development of treatments for infections and certain cancers.
