Description
Key facts
Sample types:Tissue,
Reacts with:Mouse
Product details:
Mouse on mouse polymer IHC kit ab269452 allows antibodies raised in mouse to be used on mouse tissues in IHC, without high backgrounds caused by anti-mouse secondary antibody binding to endogenous mouse antibodies in mouse tissues.
The mouse on mouse kit includes an Fab'anti-mouse IgG(H+L) blocking reagent and an HRP polymer conjugated Fab'anti-mouse IgG(H+L) detection reagent.
This kit replaces mouse on mouse kit
ab127055
. The reagents used in this kit have been carefully optimized to match the performance of
ab127055
(see validation images). This kit does not include hydrogen peroxide blocking reagent: we recommend hydrogen peroxide blocking reagent
ab64218
In the mouse on mouse IHC protocol:
- Cover the tissue section with the blocking reagent (before the addition of the mouse primary antibody) for 30-60 minutes. Wash 2-3 times.
The blocking reagent binds to endogenous mouse antibody in the tissue and prevents the binding of anti-mouse secondary antibody to those sites.
- Incubate with primary antibody of your choice raised in mouse. Wash 2-3 times.
- Incubate with HRP polymer detector reagent for ~15 minutes. Wash 2-3 times.
- Stain with a chromogen (usually DAB, eg.
ab64238
) for detection.
Otherwise, follow a conventional IHC protocol for DAB staining.
The detector and blocker have <1% reactivity to human and rabbit antibodies.
Other products for IHC
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
