Product details:
Alexa Fluor® 555 Conjugation Kit - Lightning-Link® (ab269820) uses a simple and quick process for Alexa Fluor® 555 labeling / conjugation of antibodies. It can also be used to conjugate other proteins or peptides.
To conjugate an antibody to Alexa Fluor® 555 using this kit:
- add modifier to antibody and incubate for 15 min
- add quencher and incubate for 5 min
The Alexa Fluor® 555 conjugated antibody can be used immediately in Flow cytometry, WB, ELISA, IHC etc. No further purification is required and 100% of the antibody is recovered for use.
Learn about our antibody labeling kits and their advantages
Learn about buffer compatibility below; for incompatible buffers and low antibody concentrations, use our
antibody purification and concentration kits
Custom size conjugation kits up to 100 mg are available on demand. Please contact us to discuss your requirements.
Amount and volume of antibody for conjugation to Alexa Fluor
Kit size
Recommended
amount of antibody
Maximum
amount of antibody
Maximum antibody
volume
3 x 10 µg
3 x 10 µg
3 x 20 µg
3 x 10 µL
100 µg
100 µg
200 µg
100 µL
3 x 100 µg
3 x 100 µg
3 x 200 µg
3 x 100 µL
1 x 1 mg
1 x 2 mg
1 x 1 mL
Using the maximum amount of antibody may result in less labelling per antibody.
Ideal antibody concentration is 1mg/mL. 0.5 - 1 mg/mL can be used if the maximum antibody volume is not exceeded. Antibodies > 2 mg/mL or < 0.5 mg/mL should be diluted /concentrated.
Buffer Requirements for Conjugation
Buffer should be pH 6.5-8.5.
Compatible buffer constituents
If a concentration is shown, then the constituent should be no more than the concentration shown. If several constituents are close to the limit of acceptable concentration, then this can inhibit conjugation.
50mM / 0.6% Tris
0.1% BSA
50% glycerol
0.1% sodium azide
Potassium phosphate
Sodium chloride
Sucrose
Sodium citrate
Trehalose
Tris buffered saline is almost always ≤ 50 mM / 0.6%
BSA can also interfere with the use of the conjugated antibody in tissue staining.
Incompatible buffer constituents
Thiomerosal
Proclin
Glycine
Arginine
Glutathione
If a constituent of the buffer containing your antibody or protein is not listed above, please contact us.
Only purified antibodies are suitable for use, ie. where other proteins, peptides, or amino acids are not present: antibodies in ascites fluid, serum or hybridoma culture media are incompatible.
Storing and handling conjugation kits
Lyophilized Lightning-Link
components are hygroscopic.
Kits are intentionally shipped at ambient temperature with silica gel to avoid exposure to moisture. Upon receipt, store the kit frozen and protect from moisture. Before opening the outer container, allow the lyophilized components to reach room temperature to minimize condensation.
This product is manufactured by Expedeon, an Abcam company, and was previously called Lightning-Link
Rapid Alexa Fluor
555 Labeling Kit. 333-0015 is the same as the 1 mg size. 333-0010 is the same as the 3 x 100 µg size. 333-0030 is the same as the 3 x 10 µg size. 333-0005 is the same as the 100 µg size.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or
[email protected].
Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
