Description
Key facts
Form:LiquidSee storage information,
Storage buffer:pH: 6 - 8Constituents: Agarose beads, PBS, 20% Ethanol
Product details:
His-Tag Protein Purification Column (Pre-packed, 5 x 5ml) - AminTrap (ab270529) is a simple and easy ready to use chromatography medium for purifying His-tagged proteins that have been expressed in series of expression vectors, such as E.coli., yeast, insect cells and mammalian cells.
The Ni-NTA resin contained in the column consists of 90μm beads of highly cross-linked 6% agarose, to which Nitilotriacetic acid (NTA) has been coupled. The chelating group has then been charged with nickel ions (Ni
). This form is very stable octahedral structure of nickel ions in the center, which can protect the nickel ions from attack of the competitive small molecule. The structure of Ni-NTA is compatible with a certain concentration of reducing agents, denaturing agents, detergents and other additives.
This product is manufactured by Expedeon, an Abcam company. It was previously called AminTRAP HIS Prepacked Columns 5ml - Pack of 5. Expedeon product code TNN50005 is the same as the 5 unit size of this product.
The column has a standard interface and can be adapted to all kinds of chromatography systems.
- Compatible with ÄKTA systems
- Column size: 1.6 x 2.5cm.
- Binding capacity: >40mg His-tagged protein/ml medium
- Particle size: 45-165μm.
- Max pressure: 0.3Mpa, 3 bar.
Properties and Storage Information:
Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C
