Description
Key facts
Host species:Goat,
Target species:Mouse,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Pre-adsorbed:No,
Conjugation:20nm Gold,
Applications:LFASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Polyclonal,
Isotype:IgG
Product details:
The Goat Anti-Mouse IgG antibody (GAM) is covalently conjugated to 20 nm Gold Nanoparticles which have a unique protective coating that can withstand most extreme conditions (e.g. 2.5 M NaOH at 70°C for >1 hour). The irreversible attachment does not allow the desorption of the antibody, which is a major problem with products in which the antibody is only passively adsorbed to the metal.
To exchange the conjugated antibody into a different buffer specific to your application, centrifuge the conjugate in a microfuge at 9,000 x
20 minutes. Carefully remove the supernatant, gently tap the pellet and add your preferred buffer. It is important to avoid substances that have a very high affinity for gold (e.g. thiols).
If an incubation of Goat Anti-Mouse IgG (20nm Gold, 10 OD) with the target antibody is performed, do not centrifuge the gold complex after this stage (an antibody titration is recommended).
The table below is just a guideline for possible applications:
This product is manufactured by Expedeon, an Abcam company. It was previously called 20nm InnovaCoat
GOLD (10 OD) Goat Anti Mouse IgG Conjugate. Expedeon product code 215-0200 is the same as the 200 μl size of this product and code 215-1000 is the same as the 1 mL size.
Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7 Constituents: Tris buffered saline, 0.1% Sorbitan monolaurate, ethoxylated, Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C
