Description
Key facts
Host species:Goat,
Target species:Rabbit,
Target isotype:IgG,
Target specificity:Heavy & Light chains,
Pre-adsorbed:No,
Conjugation:40nm Gold,
Applications:LFASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Clonality:Polyclonal,
Isotype:IgG
Product details:
The Goat Anti-Rabbit IgG antibody (GAR) is covalently conjugated to 40 nm Gold Nanoparticles which have a unique protective coating that can withstand most extreme conditions (e.g. 2.5 M NaOH at 70°C for >1 hour). The irreversible attachment does not allow the desorption of the antibody, which is a major problem with products in which the antibody is only passively adsorbed to the metal.
To exchange the conjugated antibody into a different buffer specific to your application, centrifuge the conjugate in a microfuge at 9,000 x
for 6 minutes. Carefully remove the supernatant, gently tap the pellet and add the preferred buffer. It is important to avoid substances that have a very high affinity for gold (e.g. thiols).
If an incubation of the conjugated Goat Anti-Rabbit IgG with the target antibody is performed, do not centrifuge the gold complex after this stage (an antibody titration is recommended).
The table below is just a guideline for possible applications:
This product is manufactured by Expedeon, an Abcam company. It was previously called 40nm InnovaCoat
GOLD (10 OD) Goat Anti Rabbit IgG Conjugate. Expedeon product code 213-0200 is the same as the 200 μl size of this product and code 213-1000 is the same as the 1 mL size.
Concentration:
10 OD. The concentration for this product is expressed in OD units. This is based on the absorbance of gold nanoparticles at 530nm of a 1:10 diluted sample.
Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7Constituents: Tris buffered saline, 0.1% Sorbitan monolaurate, ethoxylated, Shipped at conditions-Ambient - Cannot Ship with Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C
