Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Purified protein, Suspension cells, Tissue Extracts, Tissue sections, Adherent cells,
Assay type:Quantitative,
Assay Platform:Microplate
Product details:
Acyl-CoA Synthetase (ACS) Assay Kit (Fluorometric) (ab273315) provides a rapid, sensitive and straightforward way to measure ACS activity in various samples. In the assay, acyl-CoA produced by ACS activity is metabolized by the Enzyme Mix, Developer Mix and Converter Mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured with excitation at 535 nm and emission at 587 nm. This assay can detect ACS activity as low as 5 mU/μl sample.
ab273315 does not detect acyl-CoA synthetase short chain family members/acetyl-CoA synthetases.
This product is manufactured by BioVision, an Abcam company and was previously called K184 Acyl-CoA Synthetase Fluorometric Assay Kit. K184-100 is the same size as the 100 test size of ab273315.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Acyl-CoA synthetase (ACS) also known as acyl-coenzyme A synthetase is an enzyme that catalyzes the conversion of free fatty acids into acyl-CoA which is then used in various metabolic processes. It functions by activating fatty acids through a thioester bond with CoA a necessary first step in several metabolic pathways. The enzyme generally has a molecular mass around 72 kDa though this varies slightly among different isoforms. ACS enzymes are expressed in diverse tissues including the liver adipose tissue and muscle reflecting their essential role in lipid metabolism.
Biological function summary
These enzymes are critical for converting long-chain fatty acids into acyl-CoA which can then enter beta-oxidation or be used in lipid biosynthesis. ACS operates as a monomer but might be associated with multi-enzyme complexes for efficient substrate channeling. This action ensures effective regulation and partitioning of fatty acids between different cellular pathways. These enzymes play an important part in cellular energy homeostasis influencing lipid and carbohydrate metabolism.
Pathways
These enzymes play roles in fatty acid metabolism and energy production. Acyl-CoA synthetases actively participate in the beta-oxidation pathway of fatty acids a vital process for generating ATP from lipid stores. They also intersect with the synthesis of membrane lipids facilitated by proteins like acetyl-CoA carboxylase and acetyl-CoA synthetase. Their proper function ensures the balance between energy storage and consumption critical for metabolic health.
Dysregulation of ACS activity is associated with metabolic syndromes such as obesity and non-alcoholic fatty liver disease (NAFLD). Their dysfunction can disrupt normal lipid metabolism leading to abnormal fat accumulation and insulin resistance. Connections with other metabolic enzymes like acetyl-CoA carboxylase have also been observed suggesting that alterations in ACS function might influence broader metabolic networks. Understanding the role of these enzymes in such disorders could help develop targeted therapies for metabolic diseases.
