Description
Key facts
Detection method:Colorimetric,
Sample types:Tissue, Suspension cells, Adherent cells,
Assay type:Enzyme activity (quantitative),
Assay Platform:Microplate
Product details:
Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric) (ab273329) provides a quick, reliable test that allows the measurement of Fructose-1,6-Bisphosphatase (FBP) enzymatic activity in various samples. In this assay, FBP hydrolyzes Fructose-1,6-Bisphosphate into Fructose-6-Phosphate (F6P). F6P is used in an enzyme coupled reaction which reduces a chromophore forming a product with a stable signal that can measured at OD = 450 nm.
The assay is simple, sensitive and can detect lower than 100 μU of FBP in variety of samples.
This product is manufactured by BioVision, an Abcam company and was previously called K590 Fructose-1,6-Bisphosphatase Activity Assay Kit (Colorimetric). K590-100 is the same size as the 100 test size of ab273329.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Fructose-16-bisphosphatase (FBP1) and fructose-16-bisphosphatase 2 (FBP2) are enzymes that play an important role in gluconeogenesis. They catalyze the conversion of fructose-16-bisphosphate to fructose-6-phosphate an important metabolic step in glucose production. FBP1 sometimes called liver FBPase is a protein of approximately 37 kDa and it is mainly expressed in the liver kidneys and adipose tissue. FBP2 also known as muscle FBPase is primarily found in muscle tissues. These enzymes share similar enzymatic functions but differ in their regulation and tissue distribution.
Biological function summary
These enzymes help regulate glucose levels in the body. They function as part of a larger enzyme complex involved in energy homeostasis. FBP1 plays a significant role in balancing blood sugar levels during fasting by promoting gluconeogenesis in the liver. FBP2 on the other hand contributes to muscle energy management. The activity of these enzymes is tightly regulated by allosteric inhibitors and activators and they interact with other metabolic enzymes to maintain homeostasis.
Pathways
Fructose-16-bisphosphatase is involved in the gluconeogenesis pathway and is closely linked with the glycolysis pathway. In gluconeogenesis FBP1 and FBP2 convert fructose-16-bisphosphate to fructose-6-phosphate with dihydroxyacetone phosphate as an important relay. They work in tandem with other enzymes like phosphoenolpyruvate carboxykinase to facilitate glucose production. This makes them integral to metabolic pathways that provide glucose during periods of low carbohydrate intake.
Dysregulation of FBP1 and FBP2 contributes to metabolic disorders such as type 2 diabetes and hypoglycemia. Overactivity of FBP1 can lead to increased glucose production complicating glucose management in diabetes mellitus. On the other hand FBP2 mutations may impair muscle function and energy utilization. The proteins' imbalances also relate to insulin resistance as their activity influences insulin signaling pathways within metabolic disorders.
