Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Next Generation Sequencing,
Mutation description:Knockout achieved by CRISPR/Cas9 X = 1 bp insertion Frameshift: 96%,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-NR1H2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Liver X Receptor Beta (LXR beta) also known as NR1H2 is a nuclear receptor that functions as a transcription factor. It has a molecular mass of approximately 51 kDa. This receptor is primarily expressed in tissues like liver kidney adipose tissue and macrophages. LXR beta regulates genes involved in cholesterol homeostasis lipid metabolism and inflammation. Additionally LXR beta forms heterodimers with Retinoid X Receptors (RXRs) to regulate gene expression in response to ligands.
Biological function summary
The protein plays a central role in the maintenance of lipid homeostasis and modulation of inflammatory responses. LXR beta acts within a complex system that also involves other nuclear receptors and co-regulators to effectively control gene transcription. Through its activity LXR beta impacts cholesterol efflux fatty acid synthesis and inflammatory gene expression. It helps preserve cellular lipid balance a critical factor for normal cellular function and overall organism health.
Pathways
LXR beta plays a significant role in the cholesterol metabolism and lipid homeostasis pathways. It activates the transcription of genes like ABCA1 and ABCG1 important for cholesterol efflux in macrophages and other cell types. LXR beta interacts closely with proteins such as SREBP-1c (Sterol Regulatory Element Binding Protein 1c) and RXR which are integral to lipid metabolism pathways. By modulating these pathways LXR beta contributes to reverse cholesterol transport reducing cholesterol overload in cells.
LXR beta has connections to atherosclerosis and metabolic syndrome. Alterations in LXR beta function can lead to impaired cholesterol efflux and excessive lipid accumulation contributing to atherosclerosis. In metabolic syndrome irregularities in LXR beta's regulation of lipid metabolism may exacerbate the disorder's associated risks. Its interaction with proteins like GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) further highlights the target's potential role in inflammatory conditions linked to these diseases.
