Description
Key facts
Cell type:SH-SY5Y,
Species or organism:Human,
Tissue:Bone marrow,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 205 bp deletion in exon 2,
Disease:Neuroblastoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
Properties and Storage Information:
Gene name-EFNB2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ephrin B2 also called EFNB2 or ephrin-B2 is a transmembrane protein that plays important roles in cell signaling. The protein weighs approximately 37 kDa and it is expressed in various tissues like endothelial cells and neuronal tissues. Ephrin B2 binds to Eph receptors which are a part of the largest family of receptor tyrosine kinases. This interaction facilitates bidirectional signaling necessary for mediating cell communication.
Biological function summary
Ephrin-B2 serves as critical mediator for vascular development and nervous system maturation. It is an important player in angiogenesis where it functions through interaction with EphB receptors to regulate blood vessel formation and remodeling. Ephrin-B2 part of the larger ephrin family often acts in concert with other ephrins and Eph receptors to form signaling complexes that coordinate cell positioning and tissue architecture.
Pathways
The action of ephrin-B2 integrates into the Eph/ephrin signaling pathway and the VEGF signaling pathway. These pathways are important for cellular guidance and migration particularly in developing tissues. Ephrin B2's interaction with proteins like VEGF and its receptors emphasizes its role in angiogenic processes. Furthermore Ephrin-B2 interacts with other Eph receptor proteins to modulate cellular responses essential for proper vascular and neural functions.
Ephrin-B2's dysregulation associates with cancer and cardiovascular conditions. High expression of ephrin-B2 has been observed in tumor angiogenesis linking it to cancer progression through interactions with VEGF. Abnormal ephrin-B2 signaling may also relate to atherosclerosis involving disrupted endothelial cell function. These associations reveal ephrin-B2's potential as a therapeutic target for conditions involving aberrant vascular and cellular dynamics.
