Description
Key facts
Target:pCambia1300See target data,
Form:LyophilizedSee storage information,
Storage buffer:Constituents: 71% Tris, 17% EDTA
Product details:
Vector contains kanamycin resistance gene for bacterial selection, hygromycin B resistance gene for plant selection and no reporter gene to allow construction of user's own system.
The pCambia1300 vector offers:
High copy number in
E.coli
for high DNA yields
pVS1 replicon for high stability in
Agrobacterium
Small size
Restriction sites designed for modular plasmid modifications and small but adequate poly-linkers for introducing your DNA of interest
Bacterial selection with kanamycin
Plant selection with hygromycin B
Plant selection genes in the pCambia vectors are driven by a double-enhancer version of the CaMV
S promoter and terminated by the CaMV
S polyA signal.
This vector contains minimal heterologous sequences for plant transformation and selection of transformants, allowing insertion of desired genes for transformation into plants but require all promoter and terminator sequences for plant expression of newly cloned genes.
The pUC18 polylinker within the
a fragment allows blue/white screening of clones in
E. coli
cloning work.
Genbank Sequence data
Concentration of Antibiotics for selection.
In bacterial system:
Kanamycin resistance (
aphIII
nptIII
/3'5''-aminoglycoside phosphotransferase typeIII from
Enterococcus faecalis
pJH1) in bacteria: use 50 μg/mL in
E.coli
A.tumefaciens
In plant system:
Resistance Gene
(From a
Klebsiella
species, via
E.coli
Transformed plant selection antibiotic
Hygromycin B
Concentration in
Arabidopsis
50 μg/mL in germination medium
Concentration in Rice
50 μg/mL in 2N6 then in RGH6
Concentration in Tobacco
50 μg/mL in RMOP then in MST
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
