Description
Key facts
Purity:>90% SDS-PAGE,
Endotoxin level:< 1 EU/µg,
Expression system:HEK 293 cells,
Tags:His tag C-Terminus,
Applications:FuncS, SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:Measured by its binding ability in a functional ELISA.Immobilized mouse CD320-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse TCN2-His.The EC50 of biotinylated mouse TCN2-His is 0.10-0.24 μg/mL.,
Accession:Q9Z1P5,
Animal free:No,
Carrier free:No,
Species:Mouse,
Storage buffer:pH: 7.4Constituents: PBS
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The transcobalamin receptor (TCblR) also known as CD320 is a membrane protein involved in the uptake of vitamin B12 (cobalamin) by cells. TCblR is recognized for its role in the binding and internalization of transcobalamin-bound vitamin B12 enabling this essential vitamin to enter cells. The protein has a molecular mass of approximately 50 kDa. TCblR is expressed on the surface of many cell types with notable expression in bone marrow lymphoid tissues and certain tumor cells.
Biological function summary
TCblR facilitates the cellular uptake of vitamin B12 which is necessary for DNA synthesis and cell growth. It is not part of a larger protein complex but it interacts directly with transcobalamin a carrier protein that escorts vitamin B12 through the bloodstream. The interaction between TCblR and transcobalamin-bound vitamin B12 permits the transport of this vitamin into the cell where it plays a role in metabolic processes.
Pathways
TCblR plays an important role in the cobalamin metabolism pathway by mediating the internalization of vitamin B12 into cells. This uptake is important for the methionine and folate cycles where vitamin B12 functions as a cofactor. TCblR is therefore linked to the methionine synthase enzyme which is part of the folate cycle and necessary for the conversion of homocysteine to methionine.
Mutations or deficiencies in TCblR function can lead to conditions like megaloblastic anemia and neuropathy due to vitamin B12 deficiency. Altered expression of TCblR has been associated with certain types of cancer where it can influence cell proliferation by modulating vitamin B12 uptake. Its interaction with the transcobalamin protein highlights its relevance in maintaining B12 levels and cellular health.
