Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum, EDTA Plasma,
Reacts with:Chicken,
Assay type:Quantitative,
Sensitivity:= 4.5 pg/mL,
Range:4.1 - 1000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Chicken IL-21 ELISA kit (ab277406) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Chicken IL-21 in serum (Chicken IL-21 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma EDTA (Citrate and Heparin have not been validated yet) and cell culture supernatants.
This assay employs an antibody specific for Chicken IL-21 coated on a 96-well plate. Standards and samples are pipetted into the wells and Chicken IL-21 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Chicken IL-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Chicken IL-21 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
