Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.053 ng/mL,
Range:0.051 - 12.5 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human IL-28B ELISA Kit (ab277438) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-28B in serum (human IL-28B concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human IL-28B coated on a 96-well plate. Standards and samples are pipetted into the wells and Human IL-28B present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human IL-28B antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human IL-28B bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
