Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.4 ng/mL,
Range:1.229 - 300 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human OMD ELISA Kit (ab277442) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human OMD in serum (human OMD concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended as anticoagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human OMD coated on a 96-well plate. Standards and samples are pipetted into the wells and Human OMD present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human OMD antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human OMD bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Osteomodulin (OMD) also referred to as osteoadherin is a small leucine-rich proteoglycan with a molecular weight of approximately 48 kDa. This protein plays a significant role in bone biology and is primarily expressed in mineralized tissues such as osteoblasts and dentin. OMD contains multiple leucine-rich repeats that facilitate its interaction with collagen fibrils suggesting a role in the regulation of collagen assembly and matrix organization.
Biological function summary
The functions of osteomodulin contribute to maintaining bone structure and integrity. It binds to type I collagen and assists in organizing the extracellular matrix in bone tissue. OMD is not part of a larger protein complex but acts individually to stabilize the mineralized matrix. Its presence is essential for proper bone mineralization and it participates in cell signaling that governs osteogenic differentiation.
Pathways
Osteomodulin has involvement in bone development and remodeling processes. It plays a part in the TGF-beta signaling pathway which is important for bone matrix production and maintenance. OMD interacts with other matrix-related proteins including decorin and biglycan through these pathways supporting structural integrity and influencing cellular responses.
Osteomodulin's dysfunction connects to bone-related pathologies like osteoarthritis and osteoporosis. Abnormal expression of OMD can disrupt collagen regulation contributing to these conditions. It may also influence interactions with other proteins involved in these diseases such as matrix metalloproteinases further affecting tissue degradation and remodeling in diseased states.
