Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.23 ng/mL,
Range:0.2 - 150 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human PAPP A2 ELISA Kit (ab277446) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PAPP A2 in serum (human PAPP A2 concentration is low in normal serum/plasma, and may not be detectable in this assay concentration), plasma and cell culture supernatants.
This assay employs an antibody specific for Human PAPP A2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human PAPP A2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human PAPP A2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human PAPP A2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
