Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.45 ng/mL,
Range:0.41 - 100 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Galectin 10 ELISA Kit (ab277447) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human Galectin 10 in serum (human Galectin 10 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human Galectin 10 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Galectin 10 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Galectin 10 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Galectin 10 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Galectin-10 also known as Charcot-Leyden crystal protein is part of the galectin family of proteins. It functions by binding to carbohydrates due to its unique lectin properties. With a molecular mass of approximately 16.5 kDa this protein is mainly expressed in eosinophils and basophils cells that are significant in the immune system. Galectin-10 is secreted by these cells and also stored in their granules suggesting its importance in immune responses.
Biological function summary
Galectin-10 influences cellular processes like apoptosis cell adhesion and immune cell migration contributing to immune regulation. It is not well known to form part of large complexes but its release has implications on cell signaling and tissue remodeling. The presence of galectin-10 in eosinophils suggests a role in inflammation given their involvement in allergic responses and asthma.
Pathways
Studies associate galectin-10 with the allergy response and eosinophil apoptosis pathways. This protein cooperates with other galectin family members like galectin-3 in modulating cellular responses. Signaling interactions of galectin-10 impact pathways involving cell survival and death highlighting its role in immune system dynamics.
Researchers link galectin-10 to conditions such as asthma and eosinophilic disorders. Eosinophil activity in these diseases often correlates with elevated galectin-10 levels in tissues and bloodstream. Connections exist between galectin-10 and other inflammation-related proteins like interleukins influencing disease progression and severity through their regulatory roles in immune responses.
