Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 1.63 ng/mL,
Range:1.638 - 400 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Laminin alpha 4/LAMA4 ELISA Kit (ab277457) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Laminin alpha 4/LAMA4 in serum (human Laminin alpha 4 concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human Laminin alpha 4/LAMA4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Laminin alpha 4/LAMA4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Laminin alpha 4/LAMA4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Laminin alpha 4/LAMA4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Laminin alpha 4 (LAMA4) also known as laminin-8 is a subunit of the laminin protein family which are essential components of the extracellular matrix. This protein has a molecular weight of approximately 210 kDa. LAMA4 mainly expresses in the vasculature including blood vessels and organs with high metabolic rates like the heart and skeletal muscles. It plays a significant role in maintaining the structural integrity of tissues by forming a network that supports cell adhesion and migration.
Biological function summary
Laminin alpha 4 interacts with other laminin subunits such as beta and gamma chains to form heterotrimeric laminin complexes. These laminins are important for cell differentiation proliferation and tissue repair. LAMA4 influences the basement membrane dynamics contributing to the biomechanical properties of cells. Through its binding to integrins and other cell surface receptors LAMA4 activates intracellular signaling cascades necessary for organogenesis and tissue regeneration.
Pathways
Laminin alpha 4 participates actively in the PI3K/AKT pathway important for cell survival and growth. It also plays a role in the MAPK signaling pathway which is important for cell differentiation and response to stress signals. Through these pathways LAMA4 interacts with various proteins including integrins and dystroglycan modulating essential cellular processes and maintaining cellular homeostasis.
LAMA4's role becomes prominent in cancer progression where altered expression can influence tumor invasion and metastasis. Furthermore it connects to cardiovascular disorders especially in influencing angiogenesis and repair of ischemic tissues. In these contexts LAMA4 relates to proteins like integrin alpha6 and beta1 which facilitate aberrant cellular activities in disease processes. Understanding LAMA4's involvement offers potential pathways for therapeutic intervention in these diseases.
