Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 2 ng/mL,
Range:2.048 - 500 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human GSTM1 ELISA Kit (ab277475) is an
in-vitro
enzyme-linked immunosorbent assay for the quantitative measurement of Human GSTM1 in serum (human GSTM1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for Human GSTM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human GSTM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human GSTM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human GSTM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The GSTM1 protein also known as Glutathione S-transferase Mu 1 plays important roles in cellular detoxification processes. It belongs to the glutathione S-transferase family of enzymes which are involved in the conjugation of reduced glutathione to xenobiotic substrates for the purpose of detoxification. GSTM1 has a molecular mass of approximately 26 kDa. This protein expresses mostly in liver and kidney tissues but has presence in other tissues like lung and testis as well.
Biological function summary
GSTM1 acts in metabolism of endogenous and xenobiotic compounds. It does not work alone but rather functions as part of enzyme complexes that alter chemical structure of toxic substances making them safer for cellular systems. GSTM1 helps in protecting cells from damage caused by oxidative stress. The interaction of this protein with glutathione provides a protective mechanism against toxic chemicals such as carcinogens and electrophiles.
Pathways
GSTM1 has significant involvement in metabolic detoxification and the glutathione-mediated pathway. It plays a role in the metabolism of drugs and clearance of harmful compounds in these pathways. It often interacts with other proteins like GSTP1 and GSTT1 within these pathways working together to facilitate cellular defense mechanisms. GSTM1 contributes importantly to maintaining cellular redox homeostasis by its enzymatic activities.
GSTM1 shows a connection to various cancers and cardiovascular diseases. The absence or null genotype of GSTM1 associates with higher risk of developing certain cancers like bladder and lung cancer due to impaired detoxification capacity. In cardiovascular diseases the protein's deficiency can disrupt normal oxidative stress balance. GSTP1 operates alongside GSTM1 in pathological situations with their combined functions influencing disease susceptibility and progression.
