Abcam, ab28-20µg, Anti-p53 antibody [PAb 1801]

This monoclonal p53 antibody has been knockout validated in Western blot. The expected band for p53 was observed in HCT116 cells treated with irinotecan and the band was not seen in TP53 knockout HCT116 cell lysate.
We recommend using ab1101 or ab154036, alternative mouse monoclonal antibodies, to detect human p53 by IHC, ICC/IF or flow cytometry since they perform better in these applications.
Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P53 functions to control cell division and apoptosis serving as a guardian of the genome by preventing mutation accumulation. It does not form part of a larger complex under normal conditions but interacts with various other molecules to execute its functions. p53 can activate or suppress the transcription of numerous genes involved in cell cycle arrest DNA repair and programmed cell death allowing it to halt the progression of damaged cells and trigger repair mechanisms or eliminate those that cannot be repaired.
Pathways
P53 acts within several key biological pathways such as the p53 signaling pathway and the intrinsic apoptotic pathway. Its activity involves interaction with proteins like MDM2 which regulates p53 through ubiquitin-mediated degradation and ATM kinase which phosphorylates p53 in response to DNA damage. These interactions ensure appropriate cellular responses during stress and are vital for maintaining homeostasis.