Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (qualitative),
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Note: Anti-Aflibercep ELISA kit ab282907 is intended for Research Use Only, not for use in diagnostic procedures
Anti-Aflibercept ELISA kit is a sandwich ELISA assay for the qualitative detection of antibody against Aflibercept with high specificity and sensitivity in serum and plasma samples. Controls and samples are incubated in the microtiter plate coated with the drug aflibercept. After incubation, the wells are washed. Then, HRP conjugated probe is added and binds to aflibercept antibodies captured by the drug Aflibercept on the surface of the wells. Following incubation wells are washed and the bound enzymatic activity is detected by addition of chromogen-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of aflibercept antibodies in the sample or controls. The results can be evaluated with using cut-off value.
Cross Reactivity: Aflibercept infusion camouflages/masks the presence of antibody to Aflibercept in serum/plasma samples. Therefore, blood sampling time is critical for detection of anti-drug-antibodies. It is convenient to obtain blood sample just before the infusion or at least 2 weeks after the infusion of Aflibercept.
This product is manufactured by BioVision, an Abcam company and was previously called E4855 BioSim™ anti-Aflibercept (Eylea®) (Human) ELISA Kit. E4855-100 is the same size as the 1 x 96 tests size of ab282907.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
