Description
Key facts
Detection method:Colorimetric,
Sample types:Inhibitor compounds,
Assay Platform:Microplate (12 x 8 well strips)
Product details:
Angiotensin Converting Enzyme 1 (ACE1) Assay Kit (Inhibitor Screening, Colorimetric) (ab283407) can be used to screen/characterize potential inhibitors of ACE1.
The kit utilizes the ability of an active ACE1 to hydrolyze a synthetic substrate, which results in the decrease in OD at 345 nm. In the presence of Captopril, an ACE1 specific inhibitor, the ACE1 enzymatic activity is greatly reduced and there is no significant decrease in the OD at 345 nm.
The assay kit provides a rapid, simple and reliable test for high-throughput screening of ACE1 inhibitors.
Other notes
This product was previously called K719 Biovision ACE1 Inhibitor Screening Kit (Colorimetric) and Angiotensin Converting Enzyme 1 (ACE1) Inhibitor Screening Kit (Colorimetric). Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Angiotensin Converting Enzyme 1 also known as ACE1 or ACE is an important enzyme in the renin-angiotensin system. This enzyme exhibits a significant role in blood pressure regulation. ACE1 is a zinc-metallopeptidase with a molecular weight of approximately 130 kDa. It converts angiotensin I into the potent vasoconstrictor angiotensin II a critical function in cardiovascular physiology. ACE1 is widely expressed in endothelial cells particularly in the lungs kidneys and the small intestine.
Biological function summary
The enzyme generates angiotensin II by cleaving angiotensin I. Angiotensin II an important effector peptide impacts cardiovascular and renal systems influencing vasoconstriction and fluid balance. While not directly forming a complex ACE1's activity increases the potency of angiotensin II which binds to angiotensin II receptors to exert its effects therefore indirectly forming a functional signaling complex.
Pathways
ACE1 plays a central role in the renin-angiotensin system and the kallikrein-kinin system. The enzyme's activity boosts angiotensin II production which connects it to the regulation of blood pressure via the renin-angiotensin pathway. ACE1 also indirectly interacts with proteins like bradykinin by degrading them modulating kinin-related functions and influencing inflammation and tension in vascular smooth muscle.
Understanding ACE1 is important for addressing hypertension and congestive heart failure. ACE1's conversion of angiotensin I to angiotensin II means overactivity can cause elevated blood pressure leading to hypertension. This makes ACE inhibitors such as lisinopril and ramipril therapeutic for these conditions. Furthermore its connection with aldosterone production places ACE1 in relevance to heart failure as excessive aldosterone can cause detrimental remodeling of cardiac tissue.
