Description
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Form:LiquidSee storage information,
Knockout validation:Next Generation Sequencing,Western blot,
Disease:Carcinoma
Product details:
Although we aim to provide customers with a homozygous clone, feasibility will be dependent on the biology of the protein. Should only heterozygous edits be achieved, you will be notified of the outcome and be asked to confirm whether the cell line is acceptable. All clones will be accompanied with DNA sequencing data, and the mutation description.
Recommended control
: Human wild-type HCT116 cell line (ab288559). Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1.
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-EPHB2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Next Generation Sequencing, Western blot, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Eph receptor B2 also known as EphB2 is a member of the Eph receptor family which includes a number of receptor tyrosine kinases. It has a molecular weight of approximately 110 kDa. The B2 receptor is strongly expressed in various tissues including the brain spinal cord and developing embryos. These proteins include the Eph protein as well as other B2 proteins. The receptor plays a mechanical role in guiding cell positioning and tissue patterning by interacting with Ephrin ligands specifically ephrin-B2 and ephrin-B3.
Biological function summary
Eph receptor B2 functions in cell-cell communication and is involved in neuronal navigation and synaptic plasticity. It forms part of a complex that brings together ligand-receptor pairs on adjacent cells facilitating bidirectional signaling. The receptor's activation influences neuronal circuitry remodeling and helps in the differentiation of numerous cell types. The B2 protein acting as anti B2 modulates various cell signaling mechanisms essential for normal physiological functioning especially in the nervous system where it contributes to the intricate network of the eph protein interactions.
Pathways
These interactions place Eph receptor B2 in significant signaling pathways such as the MAPK/ERK and PI3K/Akt pathways. These play influential roles in cell proliferation survival and migration. The receptor interacts with numerous proteins including AB25AP through these pathways affecting developmental processes and mature cellular functions. The precise regulation of these pathways is necessary for maintaining homeostasis in complex biological systems especially within the context of the nervous system.
Alterations in Eph receptor B2 expressions have been linked to conditions such as cancer and Alzheimer’s disease. In cancers overexpression of the B2 receptor can contribute to the abnormal growth and metastasis of tumor cells often through mechanisms involving interactions with other tumorigenic proteins like Eph receptor A2. In Alzheimer's disease changes in B2 receptor signaling are associated with disrupted synaptic plasticity leading to cognitive deficits highlighting the importance of the Eph B2 receptor and its interplay with related signaling proteins in disease pathways.
