Description
Key facts
Detection method:Fluorescent,
Sample types:Adherent cells,
Reacts with:Mammals,
Assay type:Cell-based,
Assay Platform:Microplate (12 x 8 well strips)
Product details:
Fatty Acid Uptake Assay Kit (ab287857) enables rapid determination of real-time fatty acid uptake kinetics in live cells expressing fatty acid transporter proteins. The assay uses a highly fluorescent long-chain fatty acid analogue (Ex/Em = 488/523 nm) that acts as a substrate for transmembrane fatty acid transporters (FATPs) and accumulates in intracellular lipid droplets. A proprietary non-toxic membrane-impermeable quenching agent eliminates any fluorescence arising from the extracellular space, ensuring specific measurement of intracellular fatty acid accumulation without requiring any tedious wash steps. The assay is highly sensitive, has a simple "mix-and-read" protocol and is highthroughput adaptable for screening of fatty acid uptake-modulating test compounds or treatment conditions. The kit contains a complete set of reagents sufficient for performing 100 reactions in a 96-well plate format.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
