Description
Key facts
Sample types:Tissue, Suspension cells, Adherent cells
Product details:
Nuclear/Cytosol Fractionation Kit ab289882 is a complete kit that allows isolation of nuclear/cytosol fractions without ultracentrifugation.
The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contamination. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.
Nuclear/Cytosol fractionation protocol summary
- Collect cells by centrifugation
-Add Extraction buffer
- Vortex
-Incubate on Ice for 10 min
- Add lysis/cytosol extraction buffer
- Vortex
- Incubate on ice for 1 min
-Vortex then centrifuge
- Transfer the supernatant cytoplasmic extract fraction to a clean prechilled tube and place on ice
- Resuspend pellet (nuclei) and vortex and centrifuge
- Transfer the superanatant (nuclear extract) to a clean pre-chilled tube.
- Place on ice and store extract at -80°C for future use
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
