Description
Key facts
Detection method:Colorimetric,
Sample types:Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 14.5 pg/mL,
Range:14.5 - 4000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human Cathepsin E ELISA kit is an
in vitro
enzyme-linked immunosorbent assay for the quantitative measurement of human Cathepsin E in serum (plasma is not recommended in this assay) and cell culture supernatants. This assay employs an antibody specific for human Cathepsin E coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human B7-2 (CD86); BAFF R (TNFRSF13C); Calcitonin; Calsyntenin-1; cIAP-2 (HIAP-1); Coagulation Factor VII; Complement MASP3; Endocan; EphA2; EphB4; Ephrin-A4; FGF-23; FGF-5; Flt-3 (Flk-2); GLP-1 (7-37 & 7-36- NH2); Glypican 2; GM-CSF R alpha; GP73 (GOLM1); HTRA2 (Omi); IL-20 R alpha; IL-4 R alpha; JAM-C; Luteinizing Hormone (LH); Matrilin-3; Meprin alpha (MEP1A); MSP R (Ron); N-Cadherin; Neprilysin-2 (MMEL1); NKp44; Pappalysin-1 (PAPP-A); Parathyroid hormone (PTH); Pepsinogen II (PGC); Peptide YY (PYY); Presenilin 1 (PSEN1); SOX2; TFPI-2; TRACP (ACP5); Trefoil factor 3 (TFF3); Ubiquitin+1.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cathepsin E also known as CATE is an aspartic protease with a molecular mass of approximately 40 kDa. It functions within the cell to perform endopeptidase activity meaning it cleaves peptide bonds within proteins. Cathepsin E shows expression in the gastric mucosa and is prevalent in immune-related tissues such as the spleen and thymus. This enzyme operates optimally at acidic pH levels specializing in the breakdown of proteins in lysosomes.
Biological function summary
Cathepsin E plays a role in protein degradation and processing within lysosomal compartments. It associates with lysosomal and endosomal pathways participating actively in immune response regulation. Cathepsin E does not form part of a larger complex; however its activity can influence the function of other immune-related proteins by modifying antigen presentation.
Pathways
Cathepsin E engages with the major histocompatibility complex (MHC) class II pathway. This involvement assists in the processing of antigenic peptides for presentation to CD4+ T-cells connecting Cathepsin E to adaptive immune responses. Additionally it interacts with proteins such as HLA-DM which modulates the peptide loading on MHC class II molecules during immune surveillance.
Researchers link Cathepsin E to gastric cancer and Alzheimer's disease. In gastric cancer Cathepsin E overexpression can alter tumor microenvironments impacting disease progression. Furthermore in Alzheimer's disease Cathepsin E's activity correlates with amyloid precursor protein (APP) metabolism which can influence the accumulation of amyloid-beta plaques a hallmark of the disease process.
