Key facts
Detection method:Colorimetric,
Sample types:Serum, Plasma, Cell culture supernatant,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 35 pg/mL,
Range:35 - 8000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Human SDF1 beta ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human SDF1 beta in serum, plasma (human SDF1 beta concentration is low in normal serum/plasma and may not be detectable in this assay), and cell culture supernatants. This assay employs an antibody specific for human SDF1 beta coated on a 96-well plate.
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA-78, FGF-4, IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF-7, GCSF, GDNF, GM-CSF, IFN-g, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1a, MIP-1 b, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF1 alpha, TARC, TGF-b, TIMP-1, TIMP-2, TNF-a, TNF-b, TPO, VEGF.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SDF1 beta also known as CXCL12b or stromal cell-derived factor 1 beta is a small chemokine with a mass around 8 kDa. SDF1 beta plays a significant role as a signaling molecule involved in mediating various cellular responses. Expression of this chemokine occurs in a variety of tissues including spleen brain liver and skeletal muscle. Its mechanical function mainly involves binding to the CXCR4 receptor on target cells leading to the activation of signaling cascades that modulate cellular behavior.
Biological function summary
SDF1 beta participates in the regulation of immune cell trafficking and homing. As a prominent chemokine it directs leukocytes to sites where they perform their immunological duties such as inflamed or damaged tissue. Although it generally acts independently SDF1 beta can be part of a larger chemokine network collaborating with other chemokines and cytokines to orchestrate coordinated migration and positioning of immune cells.
Pathways
SDF1 beta is intricately linked with the chemokine signaling pathway and homing of hematopoietic stem cells. Activation of its pathway involves the prominent receptor CXCR4 which mediates signals that are important for the directional movement of these cells. SDF1 beta plays a central role in the hematopoiesis process where it interacts with molecules like CXCR7 which sometimes acts as a supportive player for its receptor influencing the hematopoietic stem cell niche regulation as well.
Involvement of SDF1 beta has been identified in cancer progression and inflammatory diseases like rheumatoid arthritis. In cancer it contributes to tumor growth and metastasis with CXCR4 being its main receptor partner. In rheumatoid arthritis overexpression or dysregulation of SDF1 beta influences the infiltration and retention of immune cells in the joints exacerbating inflammation. Understanding these interactions provides insights for potential therapeutic approaches targeting the SDF1 beta and CXCR4 axis in disease contexts.
