Description
Key facts
Purity:>95% HPLC,
Endotoxin level:<0.005 EU/µg,
Expression system:Escherichia coli,
Tags:His tag N-Terminus,
Applications:SDS-PAGE, Mass Spec, HPLCSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Mass Spectrometry:LC-MS/MS,
Accession:P14618,
Animal free:Yes,
Carrier free:No,
Species:Human,
Storage buffer:pH: 8Constituents: 10% Glycerol (glycerin, glycerine), 0.3002% Tris HCl
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target PKM2 also known as pyruvate kinase M2 is a type of enzyme that catalyzes the final step of glycolysis converting phosphoenolpyruvate (PEP) to pyruvate and generating ATP. The PKM2 protein has a molecular weight of about 58 kDa. PKM2 expresses in various tissues including muscles and certain cancer cells where it plays a significant role in metabolic processes. It exists in different isoforms with PKM1 being an alternative form found in pacemaker locations such as the brain and heart.
Biological function summary
PKM2 is important in cellular energy metabolism and regulates the balance between glycolysis and oxidative phosphorylation. This enzyme can exist in both dimeric and tetrameric forms with its activity level depending on its structural state. PKM2 is part of multi-protein complexes that interact with other metabolic enzymes influencing metabolic pathways that impact growth and proliferation in cells.
Pathways
PKM2 is significant in the glycolytic pathway and is also involved in the regulation of the pentose phosphate pathway. It interacts with proteins like HIF-1α a transcription factor that responds to low oxygen levels to modulate metabolic and growth pathways under hypoxic conditions. This interaction affects biosynthetic processes and energy production in rapidly dividing cells such as those in tumors.
PKM2 has a link to cancer and metabolic diseases. PKM2 is often re-expressed in cancer cells where it shifts cellular metabolism to favor rapid growth and proliferation a process known as the Warburg effect. Additionally PKM2 has connections to metabolic disorders such as type 2 diabetes where dysregulated glucose metabolism plays an important role. PKM2’s interaction with proteins like Akt and mTOR can impact signaling pathways associated with cancer progression and metabolic dysregulation.
