Description
Key facts
Detection method:Fluorometric,
Assay type:Quantitative
Product details:
Fluorescamine Protein Assay Kit (Fluorimetric) (ab324117) provides a simple 96-well or 384-well plate assay method for quantifying protein concentration in solutions.
Fluorescamine protein assay principle
In the fluorescamine protein assay, the non-fluorescent fluorescamine reacts with amine groups in proteins to form a blue-green fluorescent dye (Ex/Em 380/470 nm). The intensity of the fluorescence directly correlates with the protein concentration. Readout is with a fluorometric plate reader.
BSA (bovine serum albumin) is used to create a standard curve.
As little as 3 ug/mL of BSA can be detected.
Fluorescamine protein assay protocol summary
- add samples and standards to wells
- add fluorescamine working solution and incubate for 5 to 30 min at room temperature
- analyze with a plate reader
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
