Description
Key facts
Applications:IHC-Fr, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Form:LiquidSee storage information,
Storage buffer:Preservative: 0.1% Sodium azideConstituents: 0.3% Hydrogen peroxide
Product details:
Hydrogen Peroxide Blocking Reagent ab64218 for use in IHC with detection based on HRP / peroxidase.
Note: The concentration of hydrogen peroxide in this product is 0.3%.
IHC protocol suitable for use with Hydrogen Peroxide Blocking Reagent:
For frozen sections, skip steps 1 and 2.
1. Deparaffinize and rehydrate formalin-fixed paraffin-embedded tissue section.
2. Use appropriate
antigen retrieval buffer or enzyme
(primary antibody dependent) to treat sections. Wash 3 times in buffer.
Add enough hydrogen peroxide blocking solution ab64218 to cover the sections. Incubate for 10 minutes.
Wash 2 times in buffer. If necessary, use
avidin biotin blocking
4. Apply
protein block
normal serum
from same species as secondary antibody) and incubate for 5 minutes at room temperature to block nonspecific background staining. Wash once in buffer.
5. Apply primary antibody in
antibody diluent
and incubate.
6. Wash 4 times in buffer. Incubate slide with
biotinylated secondary antibody
HRP polymer secondary antibody
and skip step 7). Wash 4 times in buffer.
7. Apply
streptavidin-HRP
and incubate for 10 minutes at room temperature.
8. Rinse 4 times in buffer. Place slide in
DAB substrate
AEC substrate
and incubate until desired color is achieved (1-10 mins). Rinse 4 times in buffer.
9. Add enough drops of
hematoxylin
to cover the section. Incubate for 1 minute.
10. Rinse 7-8 times in tap water. Add
mounting medium
to cover the section.
Find complete IHC kits, and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the
IHC kits and reagents guide
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C
