Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Tissue Lysate, Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Sensitivity:> 0.001 mM,
Range:0.001 - 10 mM,
Assay time:40m,
Assay Platform:Microplate reader
Product details:
L-Lactate Assay Kit (Colorimetric/Fluorometric) ab65330 provides a convenient means for detecting L(+)-Lactate in biological samples such as blood, cells, culture mediums, fermentation mediums, etc. There is no need for pretreatment or purification of samples.
How the assay works
In the lactate assay protocol, lactate specifically reacts with an enzyme mix to generate a product, which interacts with a lactate probe to produce color (570 nm) and fluorescence (at Ex/Em = 535/587 nm).
Lactate assay protocol summary
- Add samples (deproteinized) and standards to wells.
- Add reaction mix and incubate for 30 min at room temp.
- Analyze with microplate reader.
Related Lactate assay products
Alternative L-Lactate assay kits offer different readout modes/wavelengths and sensitivity/range:
- This kit L-Lactate assay ab65330: colorimetric 570 nm, fluorometric Ex/Em 535/587 nm, range 0.001 mM - 10 mM.
- Our most popular L-Lactate assay kit
ab65331
: colorimetric 450nm, range 0.02 mM - 10 mM.
- L-Lactate assay kit
ab169557
: fluorometric Ex/Em 535/587 nm, range 0.2 µM - 50 µM.
L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5% of the concentration of L(+)-Lactate.
We also provide D-Lactate assay kit
ab83429
Lactate assay methods
There are two lactate assay methods established in biological research:
a) The most commonly used method, where lactate dehydrogenase processes lactate in the presence of NAD+ to produce pyruvate and NADH. NADH then reduces a probe to form a colorimetric or fluorometric readout. This method, with some improvements, is used in
ab65331
and in
ab169557
b) The alternative method, where lactate oxidase processes lactate to produce pyruvate and hydrogen peroxide. This is followed by oxidation of a substrate by a peroxidase using the hydrogen peroxide to produce a colorimetric or fluorometric readout. This method is used in ab65330.
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K607 Lactate Colorimetric/Fluorometric Assay Kit. K607-100 is the same size as the 100 test size of ab65330.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
L-Lactate also known as lactate is a byproduct of anaerobic glycolysis where it plays an important role in energy metabolism. L-Lactate is a small molecule with a molecular mass of approximately 90.08 g/mol. It forms in various tissues like muscle cells during intense exercise when oxygen levels are low. This process leads to a conversion of pyruvate to lactate by the action of the enzyme lactate dehydrogenase (LDH) which is present in many tissues with higher expressions in muscles and heart.
Biological function summary
L-Lactate acts as a signaling molecule which affects cellular functions and contributes to metabolic regulation. It is not part of a complex but serves as an important intermediate in metabolic pathways. L-Lactate provides energy to cells by converting back to pyruvate in the presence of oxygen which then enters the citric acid cycle. This conversion and its utilization as energy play important roles in balancing cellular energy demands especially under hypoxic conditions.
Pathways
L-Lactate links to critical processes like glycolysis and the Cori cycle. During glycolysis pyruvate may convert to L-Lactate under anaerobic conditions to regenerate NAD+ necessary for glycolysis to continue. In the Cori cycle lactate produced by anaerobic glycolysis in muscles is released into the bloodstream and transported to the liver. There it converts back to glucose supporting gluconeogenesis. These processes highlight the close involvement of L-Lactate with proteins such as lactate dehydrogenase and pyruvate kinase.
L-Lactate is associated with conditions like lactic acidosis and cancer. Lactic acidosis characterized by high lactate levels can occur due to oxygen deprivation or mitochondrial dysfunction. Meanwhile cancer cells often show enhanced glycolysis and lactate production known as the Warburg effect facilitating their growth. Proteins like hypoxia-inducible factor 1-alpha (HIF-1α) and lactate dehydrogenase (LDH) are key players in these conditions influencing lactate metabolism and potentially serving as therapeutic targets.
