Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine, Plasma, Cell culture supernatant, Serum, Other biological fluids, Food samples, Drinks,
Assay type:Quantitative,
Sensitivity:> 10 nM,
Range:2 - 200 µM,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
Ethanol Assay Kit ab65343 provides a simple, rapid, and sensitive method for accurate quantification of ethanol concentration in a variety of biological samples such as serum, plasma, other body fluids, foods, beverages and growth media.
In the ethanol assay protocl, alcohol oxidase oxidizes ethanol to generate H
which reacts with a probe to generate color (absorbance max 570 nm) and fluorescence (Ex/Em 535/587 nm).
The kit detects 0.1-10 ppm alcohol (~10-800 nM).
Ethanol assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min at 37°C
- analyze with microplate reader
This product is manufactured by BioVision, an Abcam company and was previously called K620 Ethanol Colorimetric/Fluorometric Assay Kit. K620-100 is the same size as the 100 test size of ab65343.
PLEASE NOTE:
Extreme care should be taken to ensure that no alcohol vapors (ethanol, methanol, propanol) are in the laboratory air where this assay is to be performed. Alcohol vapors in the air will be rapidly absorbed by kit components resulting in very high background making the kit unusable. Laboratories where HPLC equipment and solvents are standing or where alcohol is used to wipe down laboratory benches or equipment are inappropriate locations to perform this assay.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Ethanol also known as ethyl alcohol is a small volatile molecule with a molecular weight of 46.07 g/mol. Ethanol naturally occurs in the fermentation process of yeast and is present in alcoholic beverages. In cellular environments it acts ubiquitously affecting lipid membranes and interacting with various protein structures. Ethanol is not a protein but a small organic compound that exerts mechanical effects on cell membranes and proteins due to its lipophilic nature.
Biological function summary
Ethanol plays a significant role in central nervous system modulation and energy metabolism. It is not a part of a complex but affects neurotransmitter systems by interacting with ion channels and receptors such as GABA and NMDA receptors. The presence of ethanol in biological systems can disrupt normal neural signaling leading to altered mood and cognitive function which is often observed in alcohol consumption.
Pathways
Ethanol impacts several critical biological processes including the central nervous system and metabolic pathways. It influences the gamma-aminobutyric acid (GABA) pathway by enhancing inhibitory neurotransmission leading to sedative effects. Ethanol also participates in the metabolic pathways related to the conversion of alcohol to acetaldehyde and then to acetic acid involving enzymes such as alcohol dehydrogenase and aldehyde dehydrogenase. This metabolic process connects ethanol to energy production and lipid metabolism.
Ethanol consumption is closely linked to alcohol use disorders and liver disease. Chronic exposure to ethanol can lead to liver cirrhosis a condition marked by the progressive destruction of liver tissue. The interaction of ethanol with proteins like cytochrome P450 enzymes can exacerbate liver damage. Ethanol's role in altering GABA receptor activity is also associated with addiction and dependency as it enhances the inhibitory effects leading to the development of tolerance and withdrawal symptoms.
