Description
Key facts
Detection method:Colorimetric/Fluorometric,
Sample types:Urine (UTI), Plasma, Tissue Extracts, Serum, Other biological fluids, Cell Lysate,
Assay type:Quantitative,
Sensitivity:< 1 µM,
Assay time:1h,
Assay Platform:Microplate reader
Product details:
ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) use a robust, simple ATP assay method. This kit can detect as low as 1 μM of ATP in various samples
How the assay works
ATP assay kit ab83355 uses the standard established assay protocol for fluorometric and colorimetric ATP determination kits:
- glycerol is phosphorylated by glycerol kinase in an ATP dependent reaction to produce glycerol 3-phosphate
- glycerol 3-phosphate is then acted on by glycerol phosphate oxidase to produce glycerone phosphate and hydrogen peroxide
- hydrogen peroxide is then processed by a peroxidase causing a reaction with a probe to generate red color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm).
ATP assay protocol summary
- Add samples (deproteinized) and standards to wells
- Add reaction mix and incubate for 30 min at room temp
- Analyze with microplate reader
Related ATP assay products
If you require a more sensitive product, we recommend Luminescent ATP Detection Assay Kit (ab113849), which can detect as low as 1 pM of ATP.
How other researchers are using ATP Assay Kit ab83355
This ATP assay kit has been used in publications in a variety of sample types, including:
- Human: cell culture lysates
, primary monocyte cell culture lysates
, HCT116 cell culture supernatants
- Mouse: heart tissue
, liver
, C2C12 and L929 cell lysates
, primary thymocyte cell culture lysates
, cardiac tissue
- Rat: primary hippocampal neuron cell culture lysates
, liver tissue
, skeletal muscle
- Pig: kidney cell culture lysates
, heart tissue
- C. elegans tissue
- Chlamydomonas reinhardtii algae
References: 1 - Civallero M et al 2017, Na JY et al 2018, 2Gkirtzimanaki K et al 2018, 3Yang et al 2018; 4 - Singh SP et al 2018; 5 - Han SJ et al 2018; 6 - Alhindi Y et al 2019, Gregorczyk KP et al 2018; 7 - Simula L et al 2018; 8 - Litt MJ et al 2017, Samokhvalov V et al 2018; 9 - Zhao X et al 2018; 10 - Jing R et al 2018; 11 - Trinchese G et al 2018; 12 - Zou X et al 2018; 13 - Yuan F et al 2018; 14 - Pandey et al 2018; 15 - Ramanan R et al 2018
Other notes
This product is manufactured by BioVision, an Abcam company and was previously called K354 ATP Colorimetric/Fluorometric Assay Kit. K354-100 is the same size as the 100 test size of ab83355.
Cell proliferation assays are indispensable for advancing scientific discovery and clinical applications.
Browse our comprehensive selection
to support your research.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP or adenosine triphosphate acts mechanically as the key energy carrier in cellular processes. It has a molecular mass of approximately 507 Da. ATP is present throughout prokaryotic and eukaryotic cells particularly in the cytoplasm mitochondria and chloroplasts. Known also as the "molecular unit of currency" ATP transfers energy for all biochemical reactions and cellular activities. ATP functions in various cellular activities including active transport across membranes signal transduction and muscle contraction.
Biological function summary
ATP provides the energy necessary for most cellular functions. It is part of the ATP synthesis complex also known as ATP synthase localized in the mitochondrial inner membrane (in eukaryotes) and the plasma membrane (in prokaryotes). This complex facilitates ATP production through oxidative phosphorylation. ATP also plays a significant role in cellular metabolism and energy transfer. Many enzymes use ATP to catalyze reactions converting ATP to ADP and inorganic phosphate releasing energy to power cellular activities.
Pathways
ATP is integral to both glycolysis and the citric acid cycle. In glycolysis ATP participates in substrate-level phosphorylation transferring energy from glucose breakdown steps. The citric acid cycle further processes metabolic intermediates generating high-energy electron carriers that feed the electron transport chain. This chain leads to ATP production via oxidative phosphorylation. ATP interacts with enzymes like hexokinase and phosphofructokinase in glycolysis and other enzymes in the electron transport chain like cytochrome c oxidase.
ATP's role is linked to various conditions such as mitochondrial diseases and cardiac ischemia. In mitochondrial diseases mutations in mitochondrial DNA affect ATP production leading to energy deficits in cells. These disorders involve proteins like NADH dehydrogenase a component of the mitochondrial respiratory chain. In cardiac ischemia reduced oxygen supply impairs ATP synthesis affecting heart muscle contraction. Ischemia involves proteins such as creatine kinase which buffers ATP levels to sustain cellular energy during reduced blood flow.
