Description
Key facts
Purity:>95% SDS-PAGE,
Expression system:Native,
Tags:Tag free,
Applications:SDS-PAGESee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:No,
Accession:P01876,
Animal free:No,
Carrier free:No,
Species:Human,
Storage buffer:pH: 7.4Preservative: 0.05% Sodium azideConstituents: PBS, 0.87% Sodium chloride
Product details:
The Native Human IgA1 protein ab91020 is prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests. No reaction by IEP to IgA2 antiserum.
Ensure the validity of your result using our native human IgA1 protein as an isotype control in SDS-PAGE.
Check out our protein gel staining guide for SDS-PAGE
Protein Determination: Extinction Coefficient (ε) 0.1% at 280 nm, 1cm pathway = 1.32
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
IgA1 also known as Immunoglobulin A1 is an important component of the immune system. This protein mainly exists in mucous membranes where it plays an important role in immune function. IgA1 has a mass of approximately 160 kDa and is expressed in various tissues including the respiratory and gastrointestinal tracts. IgA1 is a subclass of immunoglobulin A (IgA) and is characterized by its heavy chain constant region which distinguishes it from other immunoglobulin subclasses.
Biological function summary
IgA1 acts as a first line of defense in the immune system by binding to pathogens and neutralizing them. It exists as a monomer or forms a dimer with the help of the J chain which stabilizes its dimeric form. IgA1 also interacts with the polymeric immunoglobulin receptor (pIgR) for transportation across epithelial cells. This transport allows IgA1 to be present in secretory fluids such as saliva and breast milk providing an important protective function.
Pathways
IgA1 interacts with several pathways involved in immune response and mucosal immunity. One critical pathway is the mucosal antibody response where IgA1 plays a role in preventing infection at mucosal surfaces. The IgA1 protein activates the alternative complement pathway by binding to complement protein C3 which enhances the removal of pathogens. Furthermore IgA1 is associated with the J chain and pIgR in the process of IgA transcytosis across epithelial barriers.
IgA1 has strong connections to IgA nephropathy and celiac disease. In IgA nephropathy IgA1 deposits in glomeruli leading to inflammation and kidney damage. The increased serum levels of IgA1 often with aberrant glycosylation contribute to disease development. In celiac disease IgA1 reacts with tissue transglutaminase causing an immune response that damages the small intestine's lining. In both disorders the interactions between IgA1 and other proteins such as tissue transglutaminase in celiac disease highlight its involvement in pathological processes.
