Description
Key facts
Purity:>98% SDS-PAGE,
Expression system:Insect cells,
Tags:Tag free,
Applications:FuncS, SDS-PAGE, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Biologically active:Yes,
Biological activity:The uPA activity of this product was tested using urokinase chromogenic substrate and standardized to the WHO 2nd International Standard for Human High Molecular Weight Urokinase, NIBSC 11/184. If you need the specific activity value please contact us with the lot# you require the data for.,
Accession:P06869,
Animal free:No,
Carrier free:No,
Species:Mouse,
Storage buffer:pH: 5Constituents: 0.58% Sodium chloride, 0.41% Sodium acetate, 0.0292% EDTA
Product details:
Spectrophotometric Data:
Ultraviolet Absorbance (280nm) = 0.9
epsilon
= 1.48
ab92641 demonstrates preferential binding to mouse uPAR over Human uPAR coated on an ELISA plate.
Produced using non-baculovirus insect cells.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--80°C, Appropriate long-term storage conditions--80°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Urokinase also known as urokinase-type plasminogen activator (uPA) is a serine protease with a molecular mass of approximately 54 kDa. It is expressed in various tissues including the kidneys liver and blood vessels. Urokinase initially forms as pro-urokinase an inactive precursor that can convert into the active enzyme. Researchers often use assays like the direct assay to measure urokinase activity due to its role in breaking down fibrin and modulating cell adhesion and migration.
Biological function summary
Something particularly interesting about urokinase is its role in converting plasminogen to plasmin a critical enzyme in the process of fibrinolysis. Moreover it is part of a larger enzymatic complex involving the urokinase receptor (uPAR) which localizes plasminogen activation to cell surfaces. This complex mediates extracellular matrix degradation aiding in important cellular processes like tissue remodeling and wound healing.
Pathways
Urokinase plays a critical role in the fibrinolytic pathway which involves the breakdown of blood clots. It also interacts with the extracellular matrix pathway by activating plasminogen which can degrade matrix components. In these pathways urokinase closely interacts with proteins such as plasmin and its inhibitor plasminogen activator inhibitor-1 (PAI-1) which regulate the activity and localization of proteolytic events.
Urokinase is strongly linked to conditions such as deep vein thrombosis and cancer metastasis. Its overexpression or dysregulation often leads to excessive tissue degradation influencing metastasis progression. Urokinase is connected to the urokinase receptor (uPAR) in cancer facilitating cancer cell invasion and migration. Understanding its behavior in these contexts is critical for developing therapeutic strategies and targeting treatments against these diseases.
