Description
Key facts
Species or organism:Human,
Form:LiquidSee storage information
Product details:
ab94217 is a 293T cell transfected lysate in which Human Bag1 has been transiently over-expressed using a pCMV-Bag1 plasmid. The lysate is provided in 1X Sample Buffer.
Properties and Storage Information:
Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bag1 protein also known as Bcl-2-associated athanogene 1 has a molecular mass of approximately 46 kDa. The protein is ubiquitously expressed in various tissues and cells indicating its broad functional significance. Bag1 serves as a co-chaperone interacting directly with heat shock protein 70 (Hsp70) to modulate its activity. This interaction affects the protein folding process ensuring cellular proteins maintain their proper conformation.
Biological function summary
Bag1 plays essential roles in regulating cell survival and apoptosis. It can exist as part of a larger protein complex and is known to bind with the anti-apoptotic protein Bcl-2 enhancing cell survival. By interacting with these proteins Bag1 helps control the delicate balance between cell death and survival critical for normal cellular function and response to stress. Bag1 also affects transcription regulation cell cycle progression and cellular response to stress stimuli.
Pathways
Bag1 integrates into cellular stress response and apoptotic signaling pathways. Bag1's interaction with Hsp70 associates it with the heat shock response pathway which upregulates molecular chaperones to protect cells during stress. Additionally its binding to Bcl-2 situates it within the apoptotic pathway where it supports cell survival by inhibiting caspase activation. The protein further interacts with key regulatory proteins like Raf-1 linking it to the MAPK/ERK signaling pathway involved in cell growth and division.
Dysregulation of Bag1 connects to cancer development and neurodegenerative diseases. Overexpression of Bag1 has associations with cancers such as breast cancer where it promotes tumor growth by inhibiting apoptosis. Bag1's ability to interact with the Bcl-2 protein makes it a significant player in cancer pathways. Additionally changes in Bag1 expression and function have been observed in Alzheimer's disease highlighting its role in neuron survival and stress response. Bag1's involvement with proteins like Hsp70 and Bcl-2 highlights its potential as a therapeutic target in these conditions.
