Agilent, 113001, Bond Elut C18 cartridge, LRC, 115 mg, 10 mL, 40 µm, 50/pk
Bond Elut 1cc LRC-C18, 115Mg, 50/Pk
Catalog Number: 113001
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Pack Size50/pk
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| Verified Reactivity | Mouse |
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| Antibody Type | Monoclonal |
| Host Species | Armenian Hamster |
| Immunogen | E. coli-expressed extracellular domain of the mouse TNFRI protein |
| Formulation | Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. |
| Preparation | The antibody was purified by affinity chromatography. |
| Concentration | 0.5 mg/ml |
| Storage & Handling | The antibody solution should be stored undiluted between 2°C and 8°C. |
| Application | FC - Quality testedIP, ELISA Capture, WB - Reported in the literature, not verified in house |
| Recommended Usage | Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. |
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| Application Notes |
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| Flow Cytometry3,4: For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113003/113004)or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).ELISA Capture2: The 55R-286 antibody is useful as a capture antibody when paired with the biotinylated 55R-170 antibody (Cat. No. 112904) as the detecting antibody.Additional reported applications (for the relevant formats) include: Western blotting and immunoprecipitation1. |
| Application References(PubMed link indicates BioLegend citation) | Pinckard, J.K., et al. 1997. J. Biol Chem. 272:10784. (IP) Sheehan, K.C., et al. 1995. J. Exp. Med. 181:607. (ELISA Capture) Schlaepfer, D.D., et al. 2007. J. Biol. Chem. 282:17450. (FC) Bell, J.H., et al. 2007. J. Leukocyte Biol. 82:173. (FC) Kuroishi, T., et al. 2008. J. Leukocyte Biol. 83:912. |
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| Product Citations | Mehta AK, et al. 2021. Nat Cancer. 2:66. PubMed Sun Y, et al. 2020. J Immunol. 205:3358. PubMed Janela B, et al. 2019. Immunity. 50:1069. PubMed Kuroishi T, et al. 2008. J Leukoc Biol. 83:912. PubMed |
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| RRID | AB_313530 (BioLegend Cat. No. 113001) |
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| Structure | TNFR superfamily, 55 kD |
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| Distribution | Variety of cell types at low levels |
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| Function | Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation |
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| Ligand/Receptor | TNF-α, LT-α (TNF-β) |
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| Biology Area | Immunology, Innate Immunity |
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| Molecular Family | CD Molecules, Cytokine/Chemokine Receptors |
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| Antigen References | 1. Aggarwal, B. B., et al. 1985. Nature 318 665. 2. Chan, F. K.M., et al. 2000. Science 288:2351. 3. Loetscher, H., et al. 1990. Cell 61:351. 4. Rothe, J., et al. 1993. Nature 364:798. |
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| Gene ID | 21937 |
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| UniProt | View information about CD120a on UniProt.org |
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| Clone | 55R-286 |
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| Regulatory Status | RUO |
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| Other Names | TNFR type I, TNFRI, TNFR60, p55, TNFRSF1A |
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| Isotype | Armenian Hamster IgG |
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