Agilent, 5067-5584, High Sensitivity D1000 ScreenTape
Catalog Number: 5067-5584
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| Analysis Time|MultiLine | 16 samples ˂ 20 min|96 samples ˂ 105 min| |
|---|---|
| Assay Type | High Sensitivity D1000 |
| Guaranteed Shelf Life | 6 months |
| Maximum Buffer Concentration in Sample|MultiLine | 20 mM NaOAc|20 mM guanidine-HCl|20 mM phosphate buffer|7 mM KCl|80 mM NaCl| |
| Quantitative Accuracy | ±20 % |
| Quantitative Precision | 15 % CV |
| Quantitative Range | 10-1000 pg/µL |
| Sample Volume | 2 µL |
| Sensitivity | 5 pg/µL |
| Sizing Accuracy | ± 10 % |
| Sizing Precision | 5 % CV |
| Sizing Range | 35-1000 bp |
| Sizing Resolution|MultiLine | 300-1000 bp: 10 %|35-300 bp: 15 %| |
| UNSPSC Code | 41116106 |
| Uses Per Kit | 112 |
| Uses per ScreenTape | 16 |
Answer:The assay sizes DNA from 35 to 1,000 bp and quantifies from 10 to 1,000 pg/µL, with 5 pg/µL sensitivity.
Answer:Seven ScreenTape devices provide 112 total uses, with 16 samples per device.
Answer:Use High Sensitivity D1000 Reagents 5067-5585 with this ScreenTape.
High Sensitivity D1000 ScreenTape 5067-5584 is used for automated high-sensitivity sizing and quantitation of DNA fragments from 35 to 1,000 bp on Agilent TapeStation systems. The consumable supports quality control of low-concentration DNA samples before downstream molecular-biology workflows. Each box contains seven ScreenTape devices, and each device processes 16 samples, providing 112 total uses. Pair the ScreenTape with High Sensitivity D1000 Reagents 5067-5585, which supply the ladder and sample buffer required for the assay. The workflow uses only 2 µL of sample and completes 16 samples in less than 20 minutes or 96 samples in less than 105 minutes. The assay detects DNA down to 5 pg/µL and covers a quantitative range of 10–1,000 pg/µL. Quantitative accuracy is ±20%, with 15% coefficient-of-variation precision. Sizing accuracy is ±10%, sizing precision is 5% CV, and resolution is 15% from 35 to 300 bp and 10% from 300 to 1,000 bp. Sample-buffer compatibility limits include 20 mM sodium acetate, 20 mM guanidine hydrochloride, 20 mM phosphate buffer, 7 mM potassium chloride, and 80 mM sodium chloride. The product has a guaranteed six-month shelf life and is for research use only. Low sample consumption, automated separation, defined fragment range, and fast multi-sample analysis help standardize DNA quality assessment while conserving limited libraries and PCR products.
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